Conversion of the Coenzyme Specificity of Isocitrate Dehydrogenase by Module Replacement
- 1 May 1996
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 119 (5) , 1014-1018
- https://doi.org/10.1093/oxfordjournals.jbchem.a021316
Abstract
The coenzyme specificity of isocitrate dehydrogenase from an extreme thennophilic bacterium was converted from NADP-dependent to NAD-dependent by replacing a “module” involved in the coenzyme binding site. The conversion was not possible with the replacement of a few residues that interact with the coenzyme. In addition, the modulereplaced mutant dehydrogenase was as stable as the original, wild type enzyme. The results support a previous hypothesis that a module is a structural and functional unit of a protein.Keywords
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