Multicolour whole-mount in situ hybridization to Drosophila embryos
- 1 November 1996
- journal article
- research article
- Published by Springer Nature in Wilhelm Roux' Archiv für Entwicklungsmechanik der Organismen
- Vol. 206 (4) , 292-295
- https://doi.org/10.1007/s004270050055
Abstract
We report an extended whole-mount in situ hybridization procedure for Drosophila embryos. By using probes labelled with digoxigenin, fluorescein and biotin, respectively, this protocol allows the detection in three colours of RNAs derived from three different genes. Hybridized probes are detected by consecutive staining with appropriate alkaline phosphatase conjugates using different chromogenic substrate combinations, and serial removal of the antibody conjugates by low pH washes.Keywords
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