DEMONSTRATION OF THYMUS-LEUKEMIA (TL) ANTIGENS ON MITOCHONDRIA OF LYMPHOID-CELLS BY IMMUNOELECTRON MICROSCOPY

  • 1 January 1978
    • journal article
    • research article
    • Vol. 38  (1) , 41-44
Abstract
The presence of TL antigens on the surfaces of mitochondria isolated from [mouse] TL+ cells, e.g., RADA 1 leukemia cells and TL+ normal thymocytes, was directly demonstrated by immunoelectron microscopy. Reagents used were TL alloantiserum and a hemocyanin conjugate of rabbit antibody to mouse Ig[immunoglobulin]G. No hemocyanin labeling was observed on mitochondria obtained from TL- cells, e.g., thymocytes of TL- strains, splenocytes of either TL+ or TL- strains and TL- leukemia cells. The occurrence of TL antigens on the plasma and mitochondrial membranes was shown by the ability of intact cells to absorb all reactivity toward mitochondria.

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