Single-molecule analysis of DNA-protein complexes using nanopores
- 4 March 2007
- journal article
- research article
- Published by Springer Nature in Nature Methods
- Vol. 4 (4) , 315-317
- https://doi.org/10.1038/nmeth1021
Abstract
We present a method for rapid measurement of DNA-protein interactions using voltage-driven threading of single DNA molecules through a protein nanopore. Electrical force applied to individual ssDNA-exonuclease I complexes pulls the two molecules apart, while ion current probes the dissociation rate of the complex. Nanopore force spectroscopy (NFS) reveals energy barriers affecting complex dissociation. This method can be applied to other nucleic acid–protein complexes, using protein or solid-state nanopore devices.Keywords
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