A chemiluminescent probe with a Cypridina luciferin analog, 2‐methyl‐6‐phenyl‐3,7‐dihydroimidazo[1,2‐a]pyrazin‐3‐one, specific and sensitive for O2− production in phagocytizing macrophages

Open Access
Publisher Website
Google Scholar
Abstract
When a Cypridina luciferin analog (the title compound) was added to a macrophage suspension in Hank's balanced salt solution (control), the system emitted a weak, but detectable light, which was not altered in the presence of Superoxide dismutase. The same system, however, emitted a much stronger light, just after the addition of a trigger, opsonized zymosan. The luminescence was suppressed to the control level in the presence of superoxide dismutase, while it was only slightly influenced, if at all, by NaN3, a scavenger of singlet oxygen and an inhibitor of myeloperoxidase. Some other results obtained also indicate the participation of O2 − in the luciferin analog‐dependent luminescence in macrophages during phagocytosis.

This publication has 15 references indexed in Scilit: