Isolation and partial characterization of a mutant of Penicillium funiculosum for the saccharification of straw

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Abstract
Clearing of agar plates containing ball‐milled, delignified straw has been used for screening mutants of Penicillium funiculosum IMI 87160 iii. The effects of glycerol and a number of sugars on the clearing were investigated for selecting derepressed mutants. The β‐glucosidase synthesis by one such mutant, C22c, in shake flasks containing straw was not repressed by 5% glycerol, whereas activities on filter paper, CM‐cellulose, and p‐nitrophenyl‐β‐xylosidase were only partially derepressed; xylanase was extensively derepressed. The evidence for separate control of the enzymes involved in the solubilization of straw is discussed.