Determination of acetaminophen and phenacetin in plasma by high-pressure liquid chromatography.
- 1 June 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 23 (6) , 957-959
- https://doi.org/10.1093/clinchem/23.6.957
Abstract
We describe a sensitive and precise high-pressure liquid chromatographic method in which acetoacetanilide is used as the internal standard to simultaneously determine acetaminophen and phenacetin in plasma. Therapeutic as well as toxic concentrations can be determined on as little as 0.1 ml of plasma. Sample preparation is rapid and chromatography is complete in 5 min. Quantitation is accurate at 0.5 mg/liter concentration for both drugs. Day-to-day precision within 5% is attainable. Of 36 other drugs tested, only theophylline interfered, with the determination of acetaminophen.This publication has 5 references indexed in Scilit:
- Fatal massive hepatic necrosis following acetaminophen overdoseJAMA, 1976
- High‐Pressure Liquid Chromatographic Determination of Acetaminophen in Biological FluidsJournal of Pharmaceutical Sciences, 1976
- Gas-chromatographic method for acetaminophen (N-acetyl-p-aminophenol) based on sequential alkylation.Clinical Chemistry, 1976
- Phenacetin estimation from biological samples by high-performance liquid chromatographyJournal of Chromatography A, 1976
- ESTIMATION AND IDENTIFICATION IN BLOOD-PLASMA OF PARACETAMOL (N-ACETYL-PARA-AMINOPHENOL) IN PRESENCE OF BARBITURATES1975