Identification of a Disialoganglioside (GD1a) Containing Terminal N-Acetyl-9-O-acetylneuraminic Acid in Rat Erythrocytes

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Abstract
Gangliosides containing 350 .mu.g of sialic acids were isolated from 2.85 .times. 1011 rat erythrocytes and found to be mainly composed of GD1a and an unknown alkali-labile species which was converted to GD1a after treatment with ammonia. Smaller amounts of GM1 and Fuc-GM1 were also present. Identification of the sialic acids of the novel species by TLC, high performance liquid chromatography and GLC-mass spectrometry revealed the presence of both N-acetylneuraminic acid and N-acetyl-9-O-acetylneuraminic acid in about equimolar amounts. Incubation of the isolated ganglioside with Vibrio cholerae sialidase released N-acetyl-9-O-acetylneuraminic acid. Non O-acetylated GM1 was identified as the only remaining ganglioside by TLC. Thus, this novel ganglioside has the following structure: Neu5,9Ac2.alpha.2-3Gal.beta.1-3GalNAc.beta.1-4(Neu5,Ac.alpha.-2-3)Gal.beta.1-4Glc.beta.1-1''Cer.

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