A Novel Multiplex Real-Time PCR Assay for Rapid Typing of Major Staphylococcal Cassette Chromosome mec Elements
Open Access
- 1 July 2004
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 42 (7) , 3309-3312
- https://doi.org/10.1128/jcm.42.7.3309-3312.2004
Abstract
We describe a novel procedure for rapid typing of the staphylococcal cassette chromosome mec element, a molecular marker allowing discrimination between community- and hospital-acquired methicillin-resistant Staphylococcus aureus (MRSA) strains. Oligonucleotides targeting the recombinase genes were type specific and used to type a collection of 399 MRSA isolates recovered during patient screening at admission. This novel assay constitutes a valuable tool for evaluating the molecular epidemiology of MRSA and adjusting infection control strategies against MRSA.Keywords
This publication has 21 references indexed in Scilit:
- Hospital Transmission of Community-Acquired Methicillin-Resistant Staphylococcus aureus among Postpartum WomenClinical Infectious Diseases, 2003
- Increasing Prevalence of Methicillin-Resistant Staphylococcus aureus Infection in California JailsClinical Infectious Diseases, 2003
- Identification in Methicillin-Susceptible Staphylococcus hominis of an Active Primordial Mobile Genetic Element for the Staphylococcal Cassette Chromosome mec of Methicillin-Resistant Staphylococcus aureusJournal of Bacteriology, 2003
- Comparative Molecular Analysis of Community- or Hospital-Acquired Methicillin-Resistant Staphylococcus aureusAntimicrobial Agents and Chemotherapy, 2003
- Dissemination of New Methicillin-Resistant Staphylococcus aureus Clones in the CommunityJournal of Clinical Microbiology, 2002
- Novel Type of Staphylococcal Cassette Chromosome mec Identified in Community-Acquired Methicillin-Resistant Staphylococcus aureus StrainsAntimicrobial Agents and Chemotherapy, 2002
- Association between Staphylococcus aureus strains carrying gene for Panton-Valentine leukocidin and highly lethal necrotising pneumonia in young immunocompetent patientsThe Lancet, 2002
- The Changing Epidemiology of Staphylococcus aureus?Emerging Infectious Diseases, 2001
- Effect of delayed infection control measures on a hospital outbreak of methicillin-resistant Staphylococcus aureusJournal of Hospital Infection, 2000
- Methicillin-resistant staphylococciJournal of Clinical Pathology, 1961