Ultraviolet action spectrum for intracellular free Ca2+ increase in human epidermal keratinocytes.
- 1 January 1990
- journal article
- research article
- Published by Japan Society for Cell Biology in Cell Structure and Function
- Vol. 15 (4) , 175-179
- https://doi.org/10.1247/csf.15.175
Abstract
Effects of UV on normal human epidermal keratinocytes were studied by measuring the intracellular free Ca2+ concentration ([Ca2+]i) using fluorescence ratio imaging (fura-2-AM). Upon UV irradiation the [Ca2+]i increased sharply after a certain lag time, and the UV sensitivity was higher at lower temperatures. Statistically the distribution of [Ca2+]i became broader as the mean values became larger, and the number of affected cells increased sharply above a certain fluence (light intensity .times. time [photons/cm2]) at all wavelengths studied (200-400 nm). The action spectrum showed a single peak at about 230 nm and decreased gradually toward longer-wavelength UV regions.Keywords
This publication has 1 reference indexed in Scilit:
- Ultraviolet Radiation Alters Choline Phospholipid Metabolism in Human KeratinocytesJournal of Investigative Dermatology, 1988