Release of Individual Enzymes from Guinea Pig Pancreas Following Stimulation with CCK-8 or CCK-33

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Abstract
This paper compares the effects of prolonged stimulation with CCK-33 or CCK-8 on the secretion of individual enzymes from the pancreas of the anaesthetised guinea-pig. Quantitative analysis of the individual secretory proteins was achieved using a newly developed, reversed-phase, high-performance, liquid chromatography technique. Using this system, it was possible to separate and quantify each of the nine major proteins present in a small sample of pancreatic juice in 40 min. Because spontaneous pancreatic secretion was very slow in the anaesthetised guinea-pig, the cholecystokinin (CCK) peptides were administered against a background infusion of secretin. Secretin alone produced a small rise in total protein release with the secretion of all individual proteins being increased in parallel. Doses of CCK-33 and CCK-8 which increased total protein secretion by a similar amount had contrasting effects on the release of individual proteins. CCK-33 produced a parallel release of all proteins over a period of 2 h. By contrast, after 1-h stimulation with CCK-8, the secretion of proteins became nonparallel. These data suggest that CCK-33 and CCK-8 may provoke slightly different intracellular responses within the pancreatic acinar cell.

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