Liquid chromatographic separation of cerebrospinal fluid amino acids after precolumn fluorescence derivatization
- 1 April 1989
- journal article
- research article
- Published by Hindawi Limited in Acta Neurologica Scandinavica
- Vol. 79 (4) , 273-279
- https://doi.org/10.1111/j.1600-0404.1989.tb03784.x
Abstract
Sampling and HPLC analysis procedures for CSF amino acid determination were evaluated. It is recommended to analyse native CSF. The sample should be taken from the first 12 ml obtained by lumbar puncture. Amino acid concentrations are stable for short periods over a wide range of temperature however, storage at -70.degree. to -80.degree.C is recommended. With the precolumn derivatization and a 30 cm reverse-phase column, the separation and reproducibility are reliable for analyses in the daily hospital routine.Keywords
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