ENZYMES IN ONTOGENESIS (ORTHOPTERA)

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Abstract
Eggs of the grasshopper (Melanoplus differentialis) were irradiated with a dosage of 1000 r on the 6th day after laying. This dosage destroys the embryo with no noticeable effect on the rest of the egg. Detn. of the amts. of protyrosinase, and of the natural activator of protyrosinase were made on these eggs lacking embryos. The respiration of the whole egg was also detd. The O2 consumption of the irradiated eggs increased slightly after irradiation, then fell for the remainder of the prediapause period; remained constant during diapause; and upon termination of diapause, an increase occurred for 3 days at which time the rate leveled and remained constant. The amts. of protyrosinase in control and exptl. eggs were identical. The decline with time in the amt. of activator present was the same in both groups of eggs during prediapause. During diapause the amt. of activator remained constant. The post-diapause decrease was slightly less in the irradiated eggs. Suggestions are made concerning the relations of yolk, Serosa, and embryo in the developing egg.

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