THE ROLE OF CALCIUM IONS IN FERTILIZATION OF MOUSE AND RAT EGGS IN VITRO

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Abstract
Mature female DDN mice and immature female Wistar rats were induced to superovulate and their eggs were recovered. When mouse or rat spermatozoa were incubated with eggs in Ca-free medium, the proportion of motile spermatozoa was decreased at the end of incubation. The proportion of motile spermatozoa at the end of incubation was high in the presence of Ca. No mouse eggs were penetrated in vitro in the absence of Ca2+ whether the cumulus cells were removed or not and Ca2+ appear to be necessary for the fertilization of mouse eggs in vitro in a chemically defined medium. The most suitable Ca concentration for fertilization in vitro of mouse eggs under the present experimental conditions was 1.71 mM, as in the case of Krebs-Ringer bicarbonate solution.

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