The CD45 protein tyrosine phosphatase is required for the completion of the activation program leading to lymphokine production in the Jurkat human T cell line
- 1 December 1991
- journal article
- research article
- Published by Oxford University Press (OUP) in International Immunology
- Vol. 3 (12) , 1357-1366
- https://doi.org/10.1093/intimm/3.12.1357
Abstract
Stimulation of the T cell antigen receptor, TCR-CD3, induces tyrosine phosphorylation of specific cellular proteins through activation of a tyrosine kinase. The possible regulatory role of the CD45 protein tyrosine phosphatase in this process was explored by studying the functional properties of cellular variants of the Jurkat T cell line which have been selected to have normal levels of the TCR-CD3 complex, but low or negative expression of CD45. These variants had + revertant subclone, isolated from one CD45− clone, produced normal levels of cytokines upon activation via CD3, while CD45− subclones were unable to secrete cytokines following activation via CD3. However, upon activation with Ca2+ ionophore and PMA, all CD45−(sub)clones secreted cytokines at levels comparable to those produced by CD45+ cells. These results show that CD45 is required for cytokine production after activation via the TCR-CD3 complex.Keywords
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