AN IN VITRO ASSAY FOR ESTRADIOL-17/3 AND ESTRONE1
- 1 February 1956
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 58 (2) , 150-157
- https://doi.org/10.1210/endo-58-2-150
Abstract
A rapid, sensitive, in vitro technique for the determination of estradiol-17[beta] plus estrone was presented. This estrogen assay is based on the fact that the reaction mediated by a diphos-phopyridine nucleotide (DPN) dependent isocitric dehydrogenase of human placental extracts i accelerated by these hormones. Several other steroid hormones were tested and found to have no appreciable effect on the reaction. The estradiol and estrone content of urine fractions as determined by this method was compared with results obtained by the rat vaginal smear technique; good agreement between the results of the two assays was obtained.Keywords
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