Synthesis of ppTppp via phosphotriester intermediates
Open Access
- 1 January 1983
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (21) , 7545-7554
- https://doi.org/10.1093/nar/11.21.7545
Abstract
The bifunctional and crystalline phosphorylating agent roorpholino-0,0-bis[l-benzotriazolyl]phosphate has been used for the preparation of a 3′,5′-bis-phosphotriester intermediate of thymidine. The latter has been converted Into ppTppp by the following consecutive steps; removal of the benzotriazolyl group followed by the addition of phosphoric acid and removal of the 2-(4-nitrophenyl)-ethyl group followed by the addition of pyrophosphoric acid.Keywords
This publication has 1 reference indexed in Scilit:
- A general method for the synthesis of 5'-monophosphates of DNA fragments via phosphotriester intermediatesNucleic Acids Research, 1982