AN IMPROVED CELL FRACTIONATION PROCEDURE FOR THE PREPARATION OF RAT LIVER MEMBRANE-BOUND RIBOSOMES
Open Access
- 1 January 1973
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 56 (1) , 191-205
- https://doi.org/10.1083/jcb.56.1.191
Abstract
A cell fractionation procedure is described which allows the preparation from rat liver of a rough microsome population containing almost 50% of the membrane-bound ribosomes of the tissue. The fraction is not contaminated with free ribosomes or smooth microsomes, and, by various other criteria, is suitable for studies of ribosome-membrane interaction.Keywords
This publication has 22 references indexed in Scilit:
- RIBOSOME-MEMBRANE INTERACTIONThe Journal of cell biology, 1973
- Species specific features of the distribution and multiplicity of mammalian liver catalaseArchives of Biochemistry and Biophysics, 1972
- CONTROLLED PROTEOLYSIS OF NASCENT POLYPEPTIDES IN RAT LIVER CELL FRACTIONSThe Journal of cell biology, 1970
- SUBFRACTIONATION AND COMPOSITION OF MICROSOMAL MEMBRANES: A REVIEWJournal of Histochemistry & Cytochemistry, 1968
- THE LARGE-SCALE SEPARATION OF PEROXISOMES, MITOCHONDRIA, AND LYSOSOMES FROM THE LIVERS OF RATS INJECTED WITH TRITON WR-1339The Journal of cell biology, 1968
- Studies on free and membrane-bound ribosomes in rat liverJournal of Molecular Biology, 1967
- Studies on free and membrane-bound ribosomes in rat liverJournal of Molecular Biology, 1967
- ELECTRON MICROSCOPIC EXAMINATION OF SUBCELLULAR FRACTIONSThe Journal of cell biology, 1967
- Nuclei from Rat Liver: Isolation Method That Combines Purity with High YieldScience, 1966
- The precision of ultraviolet absorption measurements in the Schmidt-Thannhauser procedure for nucleic acid estimationBiochimica et Biophysica Acta, 1962