Eriochrome Black, a Means for Reduction of Nonspecificity in Immunofluorescence

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Abstract
Fluorescent preparations with a high degree of nonspecific background staining were treated with a 1 : 60 or 1 : 30 (w/v) aqueous solution of Eriochrome black for 10 sec and then remounted. Nonspecificity was thereby considerably reduced. The technique was applied to cryostat sections of guinea pig lymph nodes containing Serratia marcescens and E. coli, with tissues of mice suffering from a pneumococcus septicemia, with Brucella-positive bovine placentas and with brain impressions of rabid foxes, mice and a cat. In all the systems studied, the fluorescent conjugate was purified only by gel filtration through Sephadex G-50, a further fractionation by ion exchange chromatography was shown to be superfluous.

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