Transcription of the promoter of the rat NF-1 gene depends on the integrity of an Sp1 recognition site.
Open Access
- 1 January 1990
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 10 (1) , 387-390
- https://doi.org/10.1128/mcb.10.1.387
Abstract
The transcription start site and promoter of the rat gene coding for the transcription factor NF-1 have been identified. The NF-1 promoter was fused to the chloramphenicol acetyltransferase-coding sequence, and the resulting plasmid was transcriptionally active in the HepG2 cell line. Footprinting and gel retardation analysis indicated that the transcription factor Sp1 binds to the NF-1 promoter. Mutants in the Sp1-binding site displayed a strong reduction in transcriptional activity.This publication has 24 references indexed in Scilit:
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