RADIOIMMUNOASSAY OF NEOCARZINOSTATIN, AN ANTITUMOR PROTEIN

  • 1 January 1976
    • journal article
    • research article
    • Vol. 36  (12) , 4378-4381
Abstract
Antibodies directed toward the antitumor protein neocarzinostatin (NCS) were produced in a rabbit by immunization with a highly purified NCS preparation. The antiserum was monospecific and reversed the antibacterial activity of NCS against Sarcina lutea. It cross reacted with chemically modified derivatives of NCS and mitomalcin but failed to cross react with macromomycin. A radioimmunoassay procedure was developed utilizing the antiserum and a biologically active 125I-labeled derivative of NCS. The lower limit of detection by this radioimmunoassay, which involves a double antibody technique for the separation of antibody bound and free antigen, was 1 .times. 10-13 mol. The sensitivity of the assay is such that serum levels of NCS can be determined accurately after administration of the drug to rats at a single dose of 2 mg/kg. Since NCS is now undergoing clinical trial, the radioimmunoassay of the drug will be a valuable tool in clinical pharmacological studies.

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