Stimulation of Pig Lymphocytes with Anti-Immunoglobulin Serum and Mitogens
- 1 January 1977
- journal article
- research article
- Published by S. Karger AG in International Archives of Allergy and Immunology
- Vol. 54 (1) , 67-77
- https://doi.org/10.1159/000231809
Abstract
Mitogenic stimulation of pig lymphocytes by anti-immunoglobulin serum and by Con A, PHA, PWM and LPS has been measured by 3H-thymidine incorporation. Lymphocytes from blood, spleen and lymph node were readily stimulated by antiglobulin. Specific anti-μ serum was mitogenic for blood lymphocytes, indicating that those B cells with surface IgM can be stimulated by complexing of this surface component with antibody. An initial 2-hour incubation with anti-Ig was as effective as continuous incubation. Differentiation of stimulated cells into high rate synthesising plasmablasts was not observed. Pig blood lymphocytes were reactive to Con A, PHA, PWM and LPS. LPS was also tested against spleen and lymph node cells, which were responsive to this mitogen.This publication has 0 references indexed in Scilit: