Identification of two factors which bind to the upstream sequences of a number of nuclear genes coding for mitochondrial proteins and to genetic elements important for cell division in yeast
- 1 January 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 16 (15) , 7287-7301
- https://doi.org/10.1093/nar/16.15.7287
Abstract
Two abundant factors GFI and GFII which interact with the 5'' flanking regions of nuclear genes conding for proteins of the mitochondrial respiratory chain have bene identified. In one case (subunit VIII of QH2: cytochrome c oxidoreductase) the binding sites for both factors overlap completely and their binding is mutually exclusive. For the other 5'' regions tested the GFI and GFII binding sites do not conicide. Interestingly, binding sites for GFI and GFII are also present in or at the 3'' ends of the coding regions of two genes of the PHO gene family and in DNA elements important for optimal ARS and CEN function respectively. The sites recognized by GFI conform to the consensus RTCRNNNNNNACGNR, while those recognized by GFII contain the element RTCACGTG. We speculate that GFI and GFII may play a role in different cellular processes, dependent on the context of their binding sites and that one of these processes mah be the coordination of the expression of genes involved in mitochondrial biogenesis with the progress of the cell cycle.This publication has 38 references indexed in Scilit:
- Yeast HAP2 and HAP3 activators both bind to the CYC1 upstream activation site, UAS2, in an interdependent mannerCell, 1987
- A yeast silencer contains sequences that can promote autonomous plasmid replication and transcriptional activationCell, 1987
- Nucleotide sequence of the gene encoding the 11‐kDa subunit of the ubiquinol – cytochrome‐c oxidoreductase in Saccharomyces cerevisiaeEuropean Journal of Biochemistry, 1987
- Yeast HAP1 activator competes with the factor RC2 for binding to the upstream activation site UAS1 of the CYC1 geneCell, 1987
- GCN4 protein, synthesize in vitro, binds HIS3 regulatory sequences: Implications for general control of amino acid biosynthetic genes in yeastCell, 1985
- Functional selection and analysis of yeast centromeric DNACell, 1985
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Structure and function of the yeast URA3 gene: expression in Escherichia coliGene, 1984
- Distinctly regulated tandem upstream activation sites mediate catabolite repression of the CYC1 gene of S. cerevisiaeCell, 1984
- Isolation, characterization and regulation of expression of the nuclear genes for the core II and Rieske iron-sulphur proteins of the yeast ubiquinol-cytochrome c reductaseGene, 1983