Phorbol ester‐induced translocation of PKC epsilon to the nucleus in fibroblasts: identification of nuclear PKC epsilon‐associating proteins
- 19 June 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 570 (1-3) , 20-24
- https://doi.org/10.1016/j.febslet.2004.05.080
Abstract
We show that phorbol ester treatment of NIH 3T3 fibroblasts induces rapid translocation of PKCε from a perinuclear site to the nucleus, extending findings in PC12 and NG108‐15 cells and in myocytes. We have immunoprecipitated the PKCε from nuclei isolated from phorbol ester‐treated fibroblasts and identified six proteins which associate with nuclear PKCε. These have been characterised as matrin 3, transferrin, Rac GTPase activating protein 1, vimentin, β‐actin and annexin II by MALDI–TOF–MS. We have confirmed that these proteins associate with PKCε by gel overlay and/or dot blotting assays. The role of these PKCε‐associating proteins in the nucleus and their interaction with PKCε are considered.Keywords
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