Synthetic Antigens as Immunogens: Part IV. Specificity of Antisera Developed to Gal β1-3(GlcNAc β1-6)GalNAc and GlcNAc βl-6GalNAc
- 1 January 1991
- journal article
- research article
- Published by Taylor & Francis in Immunological Investigations
- Vol. 20 (5-6) , 525-537
- https://doi.org/10.3109/08820139109082633
Abstract
Antisera to the chemically synthesized trisaccharide, Gal beta 1-3(GlcNAc beta 1-6)GalNAc, and disaccharide, GlcNAc beta 1-6GalNAc, were developed using the diazophenyl bovine serum albumin derivatives. The binding specificity of the antisera were analyzed by enzyme immunoassays with structurally related, chemically synthesized oligosaccharides. The anti-trisaccharide antibody showed no reactivity to T antigenic structures which bear the Gal beta 1-3GalNAc moiety. The immunodominant area of the Gal beta 1-3(GlcNAc beta 1-6)GalNAc was contained in the GlcNAc beta 1-6GalNAc region of the molecule. The reactivity pattern of the anti-trisaccharide antibody, although directed to the disaccharide, did not exactly duplicate the reactivity pattern of the anti-disaccharide.Keywords
This publication has 22 references indexed in Scilit:
- Separation by liquid chromatography (under elevated pressure) of benzyl and nitrophenyl glycosides of oligosaccharidesCarbohydrate Research, 1989
- Synthetic mucin fragments. Benzyl O-β-d-galactopyranosyl-(1→3)-O-(2-acetamido-2-deoxy-β-d-glucopyranosyl)-(1→6)-2-acetamido-2-deoxy-α-d-galactopyranoside and O-α-l-fucopyranosyl-(1→3)-O-(2-acetamido-2-deoxy-β-d-glucopyranosyl)-(1→6)-2-acetamido-2-deoxy-d-galactopyranoseCarbohydrate Research, 1989
- Structural studies on oncofetal carbohydrate antigens (Ca 19-9, Ca 50, and Ca 125) carried by O-linked sialyl-oligosaccharides on human amniotic mucinsCarbohydrate Research, 1988
- [51] GlycolipidsPublished by Elsevier ,1987
- Tumor-associated glycolipid antigens, their metabolism and organizationChemistry and Physics of Lipids, 1986
- Mucin synthesisEuropean Journal of Biochemistry, 1986
- N-acetyl-β-d-glucosaminyltransferases related to the synthesis of mucin-type glycoproteins in human ovarian tissueCarbohydrate Research, 1986
- Biosynthetic controls that determine the branching and microheterogeneity of protein-bound oligosaccharidesBiochemistry and Cell Biology, 1986
- Synthetic mucin fragments: Benzyl 2-acetamido-6-O-(2-acetamido-2-deoxy-β-d-glucopyranosyl)-2-deoxy-3-O-β-d-galactopyranosyl-α-d-galactopyranoside and benzyl 2-acetamido-6-O-(2-acetamido-2-deoxy-β-d-glucopyranosyl)-3-O-[6-O-(2-acetamido-2-deoxy-β-d-glucopyranosyl)-β- d-galactopyranosyl]-2-deoxy-α-d-galactopyranosideCarbohydrate Research, 1984
- Synthesis of 2-Acetamido-2-deoxy-3-O-(β-D-galactopyranosyl)-α-D-galactoseThe Journal of Organic Chemistry, 1965