NON-LINEAR DYNAMIC PHENOMENA IN OPEN RECONSTITUTED ENZYME-SYSTEMS

  • 1 January 1979
    • journal article
    • research article
    • Vol. 38  (11-1) , K25-K33
Abstract
The dynamic pattern of an open, reconstituted in vitro enzyme system containing [yeast] phosphofructokinase, pyruvate kinase and adenylate kinase was investigated. The approach is experimentally based on a stirred flow-through reactor. Stationary concentrations of phosphofructokinase and pyruvate kinase are maintained by entrapment in polyacrylamide gel particles. The results fit to a theoretical model based on the initial kinetic responses of the enzymes involved. An S-shaped steady state input characteristic for fructose 6-phosphate was experimentally obtained.

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