The effect of calcium on the force‐velocity relation of briefly glycerinated frog muscle fibres
- 1 October 1971
- journal article
- Published by Wiley in The Journal of Physiology
- Vol. 218 (1) , 117-145
- https://doi.org/10.1113/jphysiol.1971.sp009607
Abstract
1. Twitch fibres were isolated from the semitendinosus muscles of frogs. The sarcolemma was made more permeable by a 30 min soak in a solution containing 47·3% glycerine (v/v), 2 mM-EGTA and 10 mM phosphate buffer, pH 7. This was followed by a 30-60 min soak in a solution containing the non-ionic detergent Lubrol-WX. The fibres were then placed in a relaxing medium containing (in mM): KCl, 100; MgCl2, 1; ATP, 4; EGTA, 2; imidazole buffer, 10; pH 7·0.This publication has 29 references indexed in Scilit:
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