Measurement of Thiobarbituric Acid Value in Tissue Homogenate solubilized with Sodium Dodecylsulphate
Open Access
- 1 January 1980
- journal article
- research article
- Published by Pharmaceutical Society of Japan in YAKUGAKU ZASSHI
- Vol. 100 (5) , 553-559
- https://doi.org/10.1248/yakushi1947.100.5_553
Abstract
Determination procedures for thiobarbituric acid (TBA) reactive compounds in animal tissue homogenates were studied with sodium dodecylsulfate (SDS) as a tissue-solubilizing agent. The treatment of tissue homogenate with SDS increase the TBA value significantly; particularly, the addition of 2.5-fold SDS to protein concentration in homogenate showed maximum increase of the TBA value upon 30 min heating. The TBA increase was not at a constant rate in all tissues. The effect of SDS was not as great in young rat tissue homogenate accelerated lipid peroxidation with the treatment of ascorbate-FeCl2 or ascorbate-ADP mixture. The differences in the efficiency of SDS on TBA may depend on the physiological membrane conditions. SDS tissue-solubilizing method is useful for the exact determination of TBA in animal tissues.Keywords
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