Agrobacterium tumefaciens-mediated transformation of the monocot genus Gladiolus: detection of expression of T-DNA-encoded genes

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Abstract
Agrobacterium tumefaciens was capable of directing the transformation of Gladiolus sp., a monocot genus belonging to the family Iridaceae. Only strains capable of transferring T-DNA formed tumors, sections of which could be cultured in phytohormone-free media. Opine synthase activities were also observed in homogenates made from these tumors.