Bioluminescent Assay of Total and Brain-Specific Creatine Kinase Activity in Cerebrospinal Fluid
- 1 July 1982
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 39 (1) , 36-43
- https://doi.org/10.1111/j.1471-4159.1982.tb04698.x
Abstract
A bioluminescent assay based on the firefly luciferase reaction was used for determination of creatine kinase (CK) activity in [human] CSF. Activities as low as 0.1 U/l can be measured. The coefficient of variation at an activity level of 0.3-0.4 U/l was between 5-6%. The assay conditions optimized for serum specimens can be used for CSF. The adenylate kinase activity is almost completely inhibited, which simplifies the procedure. The CK isoenzyme distribution was obtained using the bioluminescent assay in combination with immunoinhibition or ion exchange chromatography. All specimens contained both MM [muscle type] and BB [brain type] activity, but no MB [heart type] was found. The bioluminescent assay apparently is useful in the determination of CK isoenzymes in CSF.Keywords
This publication has 22 references indexed in Scilit:
- Improved separation of creatine kinase isoenzymes by use of DEAE-Sepharose Cl-6B.Clinical Chemistry, 1980
- Creatine phosphokinase isoenzymes and the diagnosis of myocardial infarctionPostgraduate Medicine, 1980
- Assessment of cerebral injury with spinal fluid creatine kinase (CSF-CK) in patients after cardiac resuscitationScandinavian Journal of Clinical and Laboratory Investigation, 1980
- Serum creatine kinase isoenzyme MB activity: Evaluation of a kit employing agarose-gel electrophoresis with overlay paper fluorescence scanningClinica Chimica Acta; International Journal of Clinical Chemistry, 1979
- Recommended method for the determination of creatine kinase in blood modified by the inclusion of EDTA: The Committee on Enzymes of The Scandinavian Society for Clinical Chemistry and Clinical Physiology (SCE)Scandinavian Journal of Clinical and Laboratory Investigation, 1979
- Sensitive assay of creatine kinase isoenzymes in human serum using M subunit inhibiting antibody and firefly luciferaseClinica Chimica Acta; International Journal of Clinical Chemistry, 1978
- Creatine kinase in cerebrospinal fluid in newborn infantsClinica Chimica Acta; International Journal of Clinical Chemistry, 1978
- [6] Determination of creatine kinase isoenzymes in human serum by an immunological method using purified firefly luciferasePublished by Elsevier ,1978
- Adenylate Kinase Activity in Cerebrospinal Fluid in Connection with Transitory Ischaemic AttacksUpsala Journal of Medical Sciences, 1977
- Creatine phosphokinase in the cerebrospinal fluidJournal of Neurology, Neurosurgery & Psychiatry, 1967