Gas chromatography with atomic absorption detection for the determination of alkyl selenide compounds in the breath of mice after administration of different selenium compounds

Abstract
Quantitative determination of dimethylselenide and dimethyldiselenide in the breath of mice after addition of different selenium compounds to the drinking water is described. Breath samples are collected with a cryogenic trap and analyzed by gas chromatography with atomic absorption spectrometric detection. Detection limits down to 0.2 ng/100 g body weight are obtained. The pulmonary excretion of selenium metabolites after oral administration via drinking water or after intraperitoneal injection appears to be negligible. The bioconversion of the different selenium species is discussed.

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