Interaction between the TolC and AcrA Proteins of a Multidrug Efflux System of Escherichia coli
Open Access
- 15 December 2004
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 186 (24) , 8533-8536
- https://doi.org/10.1128/jb.186.24.8533-8536.2004
Abstract
This paper provides the biochemical evidence for physical interactions between the outer membrane component, TolC, and the membrane fusion protein component, AcrA, of the major antibiotic efflux pump of Escherichia coli . Cross-linking between TolC and AcrA was independent of the presence of any externally added substrate of the efflux pump or of the pump protein, AcrB. The biochemical demonstration of a TolC-AcrA interaction is consistent with genetic studies in which extragenic suppressors of a mutant TolC strain were found in the acrA gene.Keywords
This publication has 23 references indexed in Scilit:
- Genetic evidence for functional interactions between TolC and AcrA proteins of a major antibiotic efflux pump of Escherichia coliMolecular Microbiology, 2004
- Crystal Structure of the Membrane Fusion Protein, MexA, of the Multidrug Transporter in Pseudomonas aeruginosaJournal of Biological Chemistry, 2004
- Antibiotic-Sensitive TolC Mutants and Their SuppressorsJournal of Bacteriology, 2004
- AcrB Multidrug Efflux Pump of Escherichia coli : Composite Substrate-Binding Cavity of Exceptional Flexibility Generates Its Extremely Wide Substrate SpecificityJournal of Bacteriology, 2003
- Structural Basis of Multiple Drug-Binding Capacity of the AcrB Multidrug Efflux PumpScience, 2003
- Crystal structure of bacterial multidrug efflux transporter AcrBNature, 2002
- The TolC protein of Escherichia coli serves as a cell-surface receptor for the newly characterized TLS bacteriophage 1 1Edited by B. HollandJournal of Molecular Biology, 2001
- AcrA is a highly asymmetric protein capable of spanning the periplasm 1 1Edited by I. B. HollandJournal of Molecular Biology, 1999
- Two novel families of bacterial membrane proteins concerned with nodulation, cell division and transportMolecular Microbiology, 1994
- Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and MuJournal of Molecular Biology, 1976