Preparation, optimization, and structures of cross‐linked enzyme aggregates (CLEAs)
Top Cited Papers
- 19 August 2004
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 87 (6) , 754-762
- https://doi.org/10.1002/bit.20184
Abstract
The broad applicability of the cross-linking of enzyme aggregates to the effective immobilisation of enzymes is demonstrated and the influence of many parameters on the properties of the resulting CLEAs is determined. The relative simplicity of the operation ideally lends itself to high-throughput methodologies. The aggregation method was improved up to 100% activity yield for any enzyme. For the first time, the physical structures of CLEAs are elucidated.Keywords
This publication has 20 references indexed in Scilit:
- Preparative asymmetric reduction of ketones in a biphasic medium with an (S)-alcohol dehydrogenase under in situ-cofactor-recycling with a formate dehydrogenaseTetrahedron, 2004
- Immobilised enzymes: carrier-bound or carrier-free?Current Opinion in Biotechnology, 2003
- Cytosol-Mimetic Chemistry: Kinetics of the Trypsin-Catalyzed Hydrolysis of p-Nitrophenyl Acetate upon Addition of Polyethylene Glycol and N-tert-Butyl AcetoacetamideJournal of the American Chemical Society, 2003
- Active site titration as a tool for the evaluation of immobilization procedures of penicillin acylaseBiotechnology & Bioengineering, 2002
- Cross-linked aggregates of penicillin acylase: robust catalysts for the synthesis of β-lactam antibioticsJournal of Molecular Catalysis B: Enzymatic, 2001
- Cross-linked enzyme crystalsCurrent Opinion in Chemical Biology, 1999
- Novel crystalline catalystsTrends in Biotechnology, 1996
- Cross-linked enzyme crystals as robust biocatalystsJournal of the American Chemical Society, 1992
- Oxidation of non‐phenolic substratesFEBS Letters, 1990
- A novel archaebacterial NAD+‐dependent alcohol dehydrogenaseEuropean Journal of Biochemistry, 1987