Peptide elongation in rat kidney after cadmium administration
- 15 September 1980
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 190 (3) , 791-797
- https://doi.org/10.1042/bj1900791
Abstract
Rats received two injections (each 2.6 mg/kg body wt.) of CdCl2, and the kidneys were removed 24 h later. Postmicrosomal supernatant fractions of the homogenized kidneys were used as a source of elongation factors 1 and 2 in assays for [14C]phenylalanyl-tRNA binding to ribosomes and for peptide-bond synthesis. After purification of these preparations by precipitation with (NH4)2SO4 and gel filtration on Sephadex G-200 and G-100, elongation factor 1 activity was significantly increased. A significant increase in the activity of purified elongation factor 2 was also found. The results are discussed in relation to the reported effects of CdCl2 and of HgCl2 on renal tissue.This publication has 29 references indexed in Scilit:
- Degradation and re-synthesis of injected liver cadmium-thioneins in rat kidneyBiochemical Pharmacology, 1979
- Changes in rat renal cortex, isolated plasma membranes and urinary enzymes following the injection of mercuric chlorideChemico-Biological Interactions, 1977
- The biosynthesis of metallothionein in rat liver and kidney after administration of cadmiumChemico-Biological Interactions, 1976
- Aminoacyl-tRNA binding activity in regenerating kidney following contralateral nephrectomy or administration of folic acidDevelopmental Biology, 1975
- Induced synthesis of metallothionein by pig kidney cells in vitro in response to cadmiumChemico-Biological Interactions, 1975
- Factors involved in increased protein synthesis in rat kidney after administration of DDTChemico-Biological Interactions, 1974
- Muscle protein synthesis during development of the normal and dystrophic chicken Activity of supernatant elongation factorsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1974
- Mercury binding in the kidney and liver of rats repeatedly exposed to mercuric chloride: Induction of metallothionein by mercury and cadmiumToxicology and Applied Pharmacology, 1974
- Protein synthesis limited by transferase IBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1972
- Fusidic Acid: Inhibition of Factor T 2 in Reticulocyte Protein SynthesisScience, 1969