Molecular Mechanism of 5-Fluoro-2′-deoxyuridine-induced dNTP Imbalance Cell Death: Purification of an Endonuclease Involved in DNA Double Strand Breaks During dNTP Imbalance Death
- 1 November 1995
- journal article
- research article
- Published by Taylor & Francis in Nucleosides and Nucleotides
- Vol. 14 (9) , 2089-2097
- https://doi.org/10.1080/15257779508010726
Abstract
We have detected, isolated and purified an endonuclease from 5-fluoro-2′-deoxyuridine-treated FM3A cells. The molecular mass of the endonuclease was approximately 40 kDa as judged by sodium dodecyl sulfate-polyacrylamide DNA-containing gel electrophoresis. The endonuclease causes double strand breaks in DNA, with an optimum pH at 6.Keywords
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