Carboxylation Reactions of Pyruvate: Ferredoxin Oxidoreductase and 2-Oxoglutarate: Ferredoxin Oxidoreductase fromHydrogenobacter thermophilusTK-6
- 1 January 1997
- journal article
- Published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry
- Vol. 61 (3) , 510-513
- https://doi.org/10.1271/bbb.61.510
Abstract
Enzymatic reactions involving pyruvate: ferredoxin oxidoreductase and 2-oxoglutarate: ferredoxin oxidoreductase from a thermophilic, aerobic, chemolithoautotrophic, and hydrogen-oxidizing bacterium, Hydrogenobacter thermophilus TK-6, were investigated as the CO2 exchange reaction and CO2 fixation reaction using ferredoxin isolated from the same organism as a reductant. The reduced ferredoxin was required in the pyruvate synthetic reaction and the 2-oxoglutarate synthetic reaction by a cell extract that had been treated with a PD-10 column to remove the low molecular weight substances. [14C]Pyruvate and [14C]2-oxoglutarate were detected as products of pyruvate synthetic and 2-oxoglutarate synthetic reactions, respectively. Further evidence for the operation of pyruvate: ferredoxin oxidoreductase and 2-oxoglutarate: ferredoxin oxidoreductase was obtained from experiments on CO2 exchange reactions using the purified enzymes.Keywords
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