The Bacteriostatic Action of Gentian Violet and Dependence on the Oxidation-Reduction Potential

Publisher Website
Google Scholar
Abstract
Growth curves and fermentation studies showed that bacteriostatic concentrations of gentian violet affect growth only during the lag phase. Potentiometric studies showed that the dye is capable of poising the oxidation-reduction potential and thus inhibiting growth initiation. A quantitative method of measuring static action was developed and used in determining factors directly af-fecting the sensitivity of various spp. to gentian violet and other dyes. Both acidic and basic dyes are more potent in alkaline media. At optimum oxidation-reduction potential for growth initiation, dyes are least toxic. Gentian violet stimulated growth where its presence induced a more favorable potential. Other poising agents differentiate between bacterial spp., as does gentian violet. It is concluded that the bacteriostatic action of this dye is a function of its ability to poise the oxidation-reduction potential of the medium.