Establishment of a hybridoma secreting a monoclonal antibody specific for activated tumoricidal macrophages.
Open Access
- 1 October 1982
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 156 (4) , 1286-1291
- https://doi.org/10.1084/jem.156.4.1286
Abstract
New activated macrophage-specific antigen (AcM.1) detected by a monoclonal antibody was described. The AcM.1 antigen was only detectable on activated [rat and mouse] macrophages induced by pyran and Corynebacterium parvum [Propionibacterium acnes] but not on resident or thioglycollate medium (TGC), proteose-peptone and mineral oil-elicited macrophages, and not on blood monocytes and neutrophils. Activated macrophages induced by pyran and C. parvum, as expected, exhibited tumor cytotoxicity, whereas TGC-elicited macrophages did not show any cytotoxicity. Pretreatment of pyran-activated peritoneal exudate cells with AcM.1 antibody plus complement abolished .apprx. 80% cytotoxicity. This AcM.1 antigen detected by a monoclonal antibody is the 1st unique marker of activated tumoricidal macrophages and should provide a useful probe for investigating the mechanisms of activation or differentiation of macrophages.This publication has 15 references indexed in Scilit:
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