CHARACTERIZATION OF A MAJOR ANTIGENIC COMPONENT OF ASPERGILLUS-FUMIGATUS
- 1 July 1986
- journal article
- research article
- Vol. 65 (1) , 206-214
Abstract
A component of Aspergillus fumigatus Ag 7, previously identified as a major antigen for patients with allergic bronchopulmonary aspergillosis, has been isolated by gel filtration and further and purified by affinity chromatography using monospecific antiserum. The antigen, which binds both specific IgG and IgE antibodies, was shown to be a high molecular weight, 150-200 kD, heat-stable glycoprotein, which binds to concanavalin A, suggesting the presence of .alpha.-D-mannopyrannoside, or .alpha.-D-glucopyrannoside end residues. On sodiuum dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) it had a subunit of molecular weight 36 kD (with or without prior reduction), which retained antigenicity and allergenicity when tested with patients'' sera.This publication has 14 references indexed in Scilit:
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