Electrophoretic comparisons of mycelial enzymes from aflatoxin-producing and non-producing strains of Aspergillus flavus and Aspergillus parasiticus

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Abstract
Buffered mycelial extracts were prepared from 20 isolates of Aspergillus flavus Link ex. Fries and four isolates of Aspergillus parasitions Speare, including aflatoxin-producing and non-producing strains within each species. The extracts were tested for activity of various enzymes after separation by polyacrylamide gel-slab electrophoresis. Aspergillus spp. were most readily distinguished by the bands of α-esterase (EC 3.1.1.2), β-esterase (EC 3.1.1.1), and peroxidase (EC 1.11.1.7). For each isolate, the relative mobilities were determined for the various multiple-molecular forms of all three enzymes. The isolates were then compared on the basis of these relative mobilities by using a simple index of similarity to construct a single-linkage dendrogram. No distinct differences in enzyme patterns were detected between aflatoxin-producing and non-producing isolates. However, patterns of similarity or groups were indicated in the dendrogram which suggested some degree of physiological relationship.
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