Protein kinase C‐regulated production of prostacyclin by rat endothelium is increased in the presence of lipoxin A4
- 9 April 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 263 (1) , 117-120
- https://doi.org/10.1016/0014-5793(90)80718-x
Abstract
Prostacyclin is generated by cultured rat endothelial cells. Compound blocking activity of protein kinase C and cyclic nucleotide-dependent protein kinases (H7) and compound blocking interaction between Ca2+ and calmodulin (W7) diminish generation of prostacyclin in rat endothelial cells. These compounds give a synergistic effect when they are introduced to the endothelial cell cultures simultaneously. Compound HA1004, an inhibitor ofcAMP- and cGMP-dependent protein kinases has no effect on prostacyclin generation. Lipoxin A4, a potent direct stimulator of protein kinase C, rapidly induces prostacyclin generation in rat endothelium in a dose- and time-dependent fashion. Lipoxin A4-induced generation of prostacyclin can be inhibited by H7 and W7 but not by HA1004. Lipoxin B4 has no significant effect on prostacyclin generation in rat endothelium. In conclusion, our results demonstrate that generation of prostacyclin by rat endothelial cells is regulated via a pathway involving protein kinase C and Ca2+.Keywords
This publication has 16 references indexed in Scilit:
- Antigen expression in different parenchymal cell types of rat kidney and heartKidney International, 1989
- Mechanism(s) by which activation of protein kinase C is coupled to prostacyclin synthesis in granulosa cellsMolecular and Cellular Endocrinology, 1989
- Lipoxins stimulate prostacyclin generation by human endothelial cellsFEBS Letters, 1989
- Eicosanoids are regulatory molecules in γ‐interferon‐induced endothelial antigenicity and adherence for leucocytesFEBS Letters, 1989
- Control of platelet protein kinase C activation by cyclic AMPBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1988
- EVIDENCE THAT THYMECTOMIZED, BONE MARROW-RECONSTITUTED RATS DO NOT REJECT THEIR ALLOGRAFTSTransplantation, 1987
- Studies and Perspectives of Protein Kinase CScience, 1986
- Activation of protein kinase C by lipoxin A and other eicosanoids. Intracellular action of oxygenation products of arachidonic acidBiochemical and Biophysical Research Communications, 1986
- 1-(5-isoquinol inesulfonyl)-2-methylpiperazine (H-7) is a selective inhibitor of protein kinase c in rabbit plateletsBiochemical and Biophysical Research Communications, 1984
- Isoquinolinesulfonamides, novel and potent inhibitors of cyclic nucleotide-dependent protein kinase and protein kinase CBiochemistry, 1984