Preservation of Myxomycetes

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Abstract
Preservation of clonal cultures of compatible mating types of the Myxomycetes Didymium iridis and Physarum pusillum by freeze-drying, and by freezing and storing at liquid N temperatures was investigated. Subsequent to both preservation procedures viable myxamoebae were recovered. Mating ability was not impaired by either preservation treatment, as was evidenced by plasmodium formation and the production of fruiting structures with viable spores. The results suggest that 1 or both of the preservation methods may be applicable to the long-term preservation of mating and genetically characterized strains.

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