ANTI-IDIOTYPE REGULATION OF THE FORMATION OF IGE ANTIBODY TO TIMOTHY GRASS-POLLEN .2. INVITRO INDUCTION OF SUPPRESSOR T-CELLS IN MINI-MARBROOK CULTURES

  • 1 January 1983
    • journal article
    • research article
    • Vol. 48  (1) , 93-99
Abstract
A method is described for using mini-Marbrook chambers for culturing [rat] spleen cells together with antiidiotype antibody (anti-Id) to induce the appearance of suppressor T cells (Ts). Spleen cells that have been cultured with affinity prepared anti-Id (IgG), but not those cultured with normal IgG, suppress a secondary IgE response to timothy grass pollen antigen B (AgB) when injected i.v. into AgB-primed and boosted syngeneic recipient mice. Ts cells are not induced if the spleen cells cultured with anti-Id are depleted of B cells or if the cells are cultured with the F(ab)2 fragment of anti-Id; both of these results are compatible with Fc+ [Fc receptor positive] cells playing a role in the induction of Ts cells by anti-Id. Analysis of soluble suppressor factors in an ELISA [enzyme-linked immunosorbent assay] test suggests that both Ts1 and Ts2 cells may be induced by anti-Id.