The Lysis of Cholera and El Tor Vibrios

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Abstract
SUMMARY Vibrio cholerae and V. eltor underwent considerable lysis after treatment with tris + EDTA, tris + EDTA + lysozyme or sodium lauryl sulphate. Lysis induced by tris + EDTA or tris + EDTA + lysozyme was completely inhibited by 0.3 M-sucrose, 0.15 M-NaCl or 0.01 M-MgCl,. The extent of death after treatment with tris+ EDTA depended on EDTA concentration: 10 min. with 10 pg. EDTA/ml. left 43 to 50 yo of V. eltor viable and 71 to 75 % of V. cholerae. Labelled organisms exhibited maximum leakage of 32P compounds when exposed to 71 % (v/v) ethanol for V. cholerae and 50 % (v/v) ethanol for V. eltor; leakage in IOO % ethanol was about 77 yo of maximum for V. cholerae and 66% for V. eltor. Both 32P labelled and unlabelled vibrios released considerable amounts of nucleic acid, phospholipid, protein and carbohydrate and practically all their acid soluble phosphates with EDTA.

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