The “Dispersion Staining” Method for the Selective Coloration of Tissue

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Abstract
A method has been developed for the selective coloration of fixed tissue without the use of dyes. Microtome sections of formalin-fixed material are mounted under a cover glass in a mixture of 2 liquids such as diethylene glycol monobutyl ether with cinnamaldehyde and examined with the dark-field microscope. The refractive index of the liquid used for mounting must be of high dispersion and equal or close to the index of the specimen. Tissue elements, dependent on their refractive index, whether slightly lower, the same as, or slightly above the mounting medium appear colored in shades of blue, red or yellow. The optical principles involved in this optical dispersion method are similar to those involved in the production of colored light by the Christiansen filter.

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