Utility of [16 alpha-125I] iodoestradiol for autoradiography for the study of cellular and regional distribution of receptors.
Open Access
- 1 January 1987
- journal article
- abstracts
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 35 (1) , 87-92
- https://doi.org/10.1177/35.1.3794310
Abstract
We demonstrate the utility of [16 alpha-125I]iodoestradiol for thaw-mount autoradiography with 2 micron and 4 micron thick sections of rat and mouse uterus, pituitary, and brain after in vivo administration. Under the conditions of the experiments, short-term autoradiography with exposure times between 3 and 14 days provides optimal cellular resolution, whereas long-term autoradiography with 1-2 months of exposure may be used to obtain topographic-regional surveys of distribution.Keywords
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