Use of protein A-coated colloidal gold particles for immunoelectronmicroscopic localization of ACTH on ultrathin sections
- 1 January 1979
- journal article
- research article
- Published by Springer Nature in Histochemistry and Cell Biology
- Vol. 60 (3) , 317-320
- https://doi.org/10.1007/bf00500659
Abstract
ACTH was localized in dissociated porcine adenohypophysial cells using a novel indirect EM immunocytochemical technique. Incubation of ultrathin resin sections in anti-ACTH was followed by incubation with protein A-coated colloidal gold particles. Protein A binds specifically to the Fc part of the IgG molecule, and thus the ACTH-containing secretory granules became labelled with electron-dense gold particles. With this method, the dissociated porcine ACTH cell was identified as containing numerous round or ovoid 170–300 nm, secretory granules.This publication has 11 references indexed in Scilit:
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