Protective effects of calcium channel blockers on hydrogen peroxide induced increases in endothelial permeability

Publisher Website
Google Scholar
Abstract
Study objective – The aim was to examine the effects of calcium channel blockers on the permeability of endothelial cells and to determine whether these agents could protect against increases in endothelial permeability induced by hydrogen peroxide (H2O2). Design – Endothelial cells were cultured on collagen coated micropore filters. When they were confluent on the filter, albumin transfer and electrical resistance across the endothelial monolayers were measured. Experimental material – Endothelial cells were obtained from human umbilical veins. The cells at the 2nd to 4th passage were used for the experiments. Measurements and main results – Nilvadipine (10−8 M) suppressed endothelial albumin transfer by 37.2% (p−7 M), diltiazem (10−7 M), and verapamil (10−7 M) had no significant effect on either variable without the addition of H2O2. H2O2 (0.2 mM) increased albumin transfer by 164% (p−8 M) and nicardipine (10−7 M) inhibited the (0.2 mM) H2O2 induced increases in endothelial albumin transfer and decreases in electrical resistance more strongly than diltiazem and verapamil, although all of these agents significantly reduced such injury. Conclusions – Nilvadipine is a potent inhibitor of endothelial permeability and of hydrogen peroxide induced increases in permeability.

This publication has 0 references indexed in Scilit: