A multimember kinesin gene family in Drosophila.
- 15 May 1991
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 88 (10) , 4424-4427
- https://doi.org/10.1073/pnas.88.10.4424
Abstract
Degenerate primers to the kinesin motor domain were used in the polymerase chain reaction to amplify DNA sequences from Drosophila genomic DNA and cDNA libraries. The amplified DNA sequences were hybridized to polytene chromosomes and the map positions of the hybridizing sites were determined. More than 30 sites of hybridization were detected, indicating that the kinesin gene family may be much larger than previously thought. One new family member has already been identified as a result of this screen. The map positions should aid in the identification of further kinesin family members. Some of these kinesin-related genes are anticipated to function in previously undiscovered roles in the cell.Keywords
This publication has 20 references indexed in Scilit:
- The C. elegans unc-104 4 gene encodes a putative kinesin heavy chain-like proteinNeuron, 1991
- The kinesin-like ncd protein of Drosophila is a minus end-directed microtubule motorCell, 1990
- The Drosophila claret segregation protein is a minus-end directed motor moleculeNature, 1990
- Novel potential mitotic motor protein encoded by the fission yeast cut7+ geneNature, 1990
- A kinesin-like protein required for distributive chromosome segregation in DrosophilaCell, 1990
- Identification and characterization of a gene encoding a kinesin-like protein in DrosophilaCell, 1990
- Mutation of a gene that encodes a kinesin-like protein blocks nuclear division in A. nidulansCell, 1990
- A three-domain structure of kinesin heavy chain revealed by DNA sequence and microtubule binding analysesCell, 1989
- Functional cDNA libraries from Drosophila embryosJournal of Molecular Biology, 1988
- A technique for radiolabeling DNA restriction endonuclease fragments to high specific activityAnalytical Biochemistry, 1984