The Istar 1 Serodeme of Trypanosoma brucei: Development of a New Serodeme

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Abstract
An extensive serodeme of sequentially-isolated antigenic variants of African trypanosomes has been produced from both syringe-passaged and cyclically-transmitted T. brucei of the IsTaR 1 clone derived from EATRO 164. The majority of the antigenic variants were isolated from chronically-infected deer mice (Peromyscus leucopus). The pattern of parasitemias during the course of infections initiated with syringe-passaged trypanosomes differed from those initiated with cyclically-transmitted trypanosomes. Trypanosome populations from syringe-passed (192) and cyclically-transmitted (31) clones were each amplified by growth in lethally-irradiated mice and cryopreserved for retrospective analysis. Five clones derived from a single deer mouse during the 1st 44 days of infection, and 2 clones derived from an acutely infected rat were established from these amplified populations. Homogeneous populations were grown in lethally irradiated rats and mice for antigenic analysis purification of variant-specific glycoproteins. Of the 7 clones, 6 were distant variants by immunological criteria using antisera derived from whole cells or purified surface glycoproteins. Two clones, one derived from the acutely infected rat, and the other from the first parasitemia in a chronic infection that was initiated with the former clone, were immunologically identical. Production of these clones established a well defined serodeme that wil allow detailed analysis of antigenic variation.